# Benjamin Gaudio ![ben_thumbnail](/sites/g/files/omnuum5326/files/styles/hwp_1_1__360x360_scale/public/bengaudio/files/thumbnail_ben_gaudio_sog_0027_20.png?itok=5nWmhkAn) ## About Ben: Ben recieved his Bachelor's of Science in Biological Sciences from Northeastern University in 2018 and completed his Master's degree at Harvard Extension School in 2024. As part of the Sorger lab, Ben has worked on the LINCS project creating dose response profiles of a large number of cancer cell lines, helped create a new assay for detecting SARS-CoV-2 which causes COVID19, developed numerous CRISPR cell line models to probe the biology of cancer cells and investigate cellular transport mechanisms, and contributed to the development of the CyCIF imaging platform. Currently, he works on improving spatial imaging platforms and is involved in projects focused on 3D microscopy and automation of highly-multiplexed fluorescent imaging. ## Recent Publications Download 5 citations download- [BibTeX](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=bibtex) - [EndNote X3 XML](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=endnote8) - [EndNote 7 XML](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=endnote7) - [Endnote tagged](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=tagged) - [Marc](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=marc) - [PubMedId](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=pubmed_id) - [RIS](/bibcite/export?pager_style=no_pager&number_of_items=5&sort_field=bibcite_year--desc&taxonomy_filters=&&&format=ris) ### In Preparation Marian Kalocsay, Matthew J. Berberich, Robert A. Everley, Maulik K. Nariya, Mirra Chung, Ben Gaudio, Chiara Victor, Gary A. Bradshaw, Marc Hafner, Peter K. Sorger, Caitlin E.Mills, and Kartik Subramanian. “[Proteomic Profiling of Breast Cancer Cell Lines and Models](/publications/proteomic-profiling-breast-cancer-cell-lines-and-models)” Marian Kalocsay, Matthew J. Berberich, Robert A. Everley, Maulik K. Nariya, Mirra Chung, Ben Gaudio, Chiara Victor, Gary A. Bradshaw, Marc Hafner, Peter K. Sorger, Caitlin E.Mills, and Kartik Subramanian. “[Proteomic Profiling of Breast Cancer Cell Lines and Models](/publications/proteomic-profiling-breast-cancer-cell-lines-and-models)” - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://doi.org/10.1101/2020.12.15.422823) We performed quantitative proteomics on 61 human-derived breast cancer cell lines to a depth of ~13,000 proteins. The resulting high-throughput datasets were assessed for quality and reproducibility. We used the datasets to identify and characterize the... - [ descriptionPublisher's Version](https://doi.org/10.1101/2020.12.15.422823) ### 2024 Christopher Chidley, Alicia M. Darnell, Benjamin L. Gaudio, Evan C. Lien, Anna M. Barbeau, Matther G. Vander Heiden, and Peter K. Sorger. 2024. “[A CRISPRi A Screening Platform to Study Cellular Nutrient Transport in Diverse Microenvironments](/publications/crispria-screening-platform-study-cellular-nutrient-transport-diverse)”. Nature Cell Biology, 26, Pp. 825-38 Christopher Chidley, Alicia M. Darnell, Benjamin L. Gaudio, Evan C. Lien, Anna M. Barbeau, Matther G. Vander Heiden, and Peter K. Sorger. 2024. “[A CRISPRi A Screening Platform to Study Cellular Nutrient Transport in Diverse Microenvironments](/publications/crispria-screening-platform-study-cellular-nutrient-transport-diverse)”. Nature Cell Biology, 26, Pp. 825-38 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://www.nature.com/articles/s41556-024-01402-1) Blocking the import of nutrients essential for cancer cell proliferation represents a therapeutic opportunity, but it is unclear which transporters to target. Here we report a CRISPR interference/activation screening platform to systematically interrogate... - [ descriptionPublisher's Version](https://www.nature.com/articles/s41556-024-01402-1) ### 2022 Caitlin E.Mills, Kartik Subramanian, Marc Hafner, Mario Niepel, Luca Gerosa, Mirra Chung, Chiara Victor, Benjamin Gaudio, Clarence Yapp, Ajit J. Nirmal, Nicholas Clark, and Peter K. Sorger. 2022. “[Multiplexed and Reproducible High Content Screening of Live and Fixed Cells Using Dye Drop](/publications/multiplexed-and-reproducible-high-content-screening-live-and-fixed-cells)”. Nature Communications, 13, 6918 Caitlin E.Mills, Kartik Subramanian, Marc Hafner, Mario Niepel, Luca Gerosa, Mirra Chung, Chiara Victor, Benjamin Gaudio, Clarence Yapp, Ajit J. Nirmal, Nicholas Clark, and Peter K. Sorger. 2022. “[Multiplexed and Reproducible High Content Screening of Live and Fixed Cells Using Dye Drop](/publications/multiplexed-and-reproducible-high-content-screening-live-and-fixed-cells)”. Nature Communications, 13, 6918 - [ descriptionPublisher's Version](https://doi-org.ezp-prod1.hul.harvard.edu/10.1038/s41467-022-34536-7) - [ descriptionPublisher's Version](https://doi-org.ezp-prod1.hul.harvard.edu/10.1038/s41467-022-34536-7) ### 2020 Jason Qian, Sarah A. Boswell, Christopher Chidley, Zhi-xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, Ryan T. Ingram, Rebecca H. Ward, Jonathan Z. Li, and Michael Springer. 2020. “[An Enhanced Isothermal Amplification Assay for Viral Detection](/publications/enhanced-isothermal-amplification-assay-viral-detection)”. Nature Communications Jason Qian, Sarah A. Boswell, Christopher Chidley, Zhi-xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, Ryan T. Ingram, Rebecca H. Ward, Jonathan Z. Li, and Michael Springer. 2020. “[An Enhanced Isothermal Amplification Assay for Viral Detection](/publications/enhanced-isothermal-amplification-assay-viral-detection)”. Nature Communications - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](https://doi.org/10.1101/2020.05.28.118059) Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. We developed a molecular diagnostic test... - [ descriptionPublisher's Version](https://doi.org/10.1101/2020.05.28.118059) ### 2019 Mario Niepel, Marc Hafner, Caitlin E.Mills, Kartik Subramanian, Elizabeth H. Williams, Mirra Chung, Benjamin Gaudio, and Anne Marie Barrett. 2019. “[A Multi-Center Study on the Reproducibility of Drug-Response Assays in Mammalian Cell Lines](/publications/multi-center-study-reproducibility-drug-response-assays-mammalian-cell-lines)”. Cell Systems, 9, 1, Pp. 35-48 Mario Niepel, Marc Hafner, Caitlin E.Mills, Kartik Subramanian, Elizabeth H. Williams, Mirra Chung, Benjamin Gaudio, and Anne Marie Barrett. 2019. “[A Multi-Center Study on the Reproducibility of Drug-Response Assays in Mammalian Cell Lines](/publications/multi-center-study-reproducibility-drug-response-assays-mammalian-cell-lines)”. Cell Systems, 9, 1, Pp. 35-48 - add\_circle do\_not\_disturb\_on Abstract - [ descriptionPublisher's Version](http://dx.doi.org/10.1016/j.cels.2019.06.005) Evidence that some high-impact biomedical results cannot be repeated has stimulated interest in practices that generate findable, accessible, interoperable, and reusable (FAIR) data. Multiple papers have identified specific examples of irreproducibility... - [ descriptionPublisher's Version](http://dx.doi.org/10.1016/j.cels.2019.06.005) [ More arrow\_circle\_right ](/publications)